Depending on the degree of disinfection that you are looking to accomplish, the dilution rate will change.
For daily cleaning and disinfection, we recommend the 1:64 dilution (2 oz/gallon of water), with a contact time of 5 minutes.
In situations where you suspect or have confirmed an infectious disease outbreak, we recommend the 1:16 dilution (8 oz/gallon of water) with a contact time of 5 minutes.
You may also find these resources useful:
Video: Rescue Concentrate - How to Dilute
Video: How to Use Rescue Concentrate