Yes, Rescue™ is effective against Mycoplasma.
With the Rescue™ Ready-to-Use Liquid and Wipes, we recommend a contact time of 1 minute.
With Rescue™ Concentrate, we recommend using the 1:16 dilution for a contact time of 5 minutes, or a dilution of 1:64 with a contact time of 10 minutes.
Contact time is the amount of time the liquid should remain wet on the surface in order to achieve disinfection.
You may also find these resources useful:
Video: Avoid Aerosolization
Video: How to Clean and Disinfect a Cage
Video: How to Clean and Disinfect an Exam Room